SARS-CoV-2 Spike Protein-Expressing Enterococcus for Oral Vaccination: Immunogenicity and Protection.

The declaration of the conclusion of the COVID-19 pandemic notwithstanding, coronavirus remains prevalent in circulation, and the potential emergence of novel variants of concern introduces the possibility of new outbreaks. Moreover, it is not clear how quickly and to what extent the effectiveness of vaccination will decline as the virus continues to mutate. One possible solution to combat the rapidly mutating coronavirus is the creation of safe vaccine platforms that can be rapidly adapted to deliver new, specific antigens in response to viral mutations. Recombinant probiotic microorganisms that can produce viral antigens by inserting specific viral DNA fragments into their genome show promise as a platform and vector for mucosal vaccine antigen delivery. The authors of this study have developed a convenient and universal technique for inserting the DNA sequences of pathogenic bacteria and viruses into the gene that encodes the pili protein of the probiotic strain E. faecium L3. The paper presents data on the immunogenic properties of two E. faecium L3 vaccine strains, which produce two different fragments of the coronavirus S1 protein, and provides an assessment of the protective efficacy of these oral vaccines against coronavirus infection in Syrian hamsters.

Estimation of anti-orthopoxvirus immunity in Moscow residents and potential risks of spreading Monkeypox virus.

WHO has declared the outbreak of monkeypox as a public health emergency of international concern. In less than three months, monkeypox was detected in more than 30 000 people and spread to more than 80 countries around the world. It is believed that the immunity formed to smallpox vaccine can protect from monkeypox infection with high efficiency. The widespread use of Vaccinia virus has not been carried out since the 1980s, which raises the question of the level of residual immunity among the population and the identification of groups requiring priority vaccination. We conducted a cross-sectional serological study of remaining immunity among Moscow residents. To do this, a collection of blood serum samples of age group over 30 years old was formed, an in-house ELISA test system was developed, and a virus neutralization protocol was set up. Serum samples were examined for the presence of IgG antibodies against Vaccinia virus (n=2908), as well as for the ability to neutralize plaque formation with a Vaccinia virus MNIIVP-10 strain (n=299). The results indicate the presence of neutralizing antibody titer of 1/20 or more in 33.3 to 53.2% of people older than 45 years. Among people 30-45 years old who probably have not been vaccinated, the proportion with virus neutralizing antibodies ranged from 3.2 to 6.7%. Despite the higher level of antibodies in age group older than 66 years, the proportion of positive samples in this group was slightly lower than in people aged 46-65 years. The results indicate the priority of vaccination in groups younger than 45, and possibly older than 66 years to ensure the protection of the population in case of spread of monkeypox among Moscow residents. The herd immunity level needed to stop the circulation of the virus should be at least 50.25 - 65.28%.

Antiviral Activity of Umifenovir In Vitro against a Broad Spectrum of Coronaviruses, Including the Novel SARS-CoV-2 Virus.

An escalating pandemic of the novel SARS-CoV-2 virus is impacting global health, and effective antivirals are needed. Umifenovir (Arbidol) is an indole-derivative molecule, licensed in Russia and China for prophylaxis and treatment of influenza and other respiratory viral infections. It has been shown that umifenovir has broad spectrum activity against different viruses. We evaluated the sensitivity of different coronaviruses, including the novel SARS-CoV-2 virus, to umifenovir using in vitro assays. Using a plaque assay, we revealed an antiviral effect of umifenovir against seasonal HCoV-229E and HCoV-OC43 coronaviruses in Vero E6 cells, with estimated 50% effective concentrations (EC50) of 10.0 ± 0.5 µM and 9.0 ± 0.4 µM, respectively. Umifenovir at 90 µM significantly suppressed plaque formation in CMK-AH-1 cells infected with SARS-CoV. Umifenovir also inhibited the replication of SARS-CoV-2 virus, with EC50 values ranging from 15.37 ± 3.6 to 28.0 ± 1.0 µM. In addition, 21-36 µM of umifenovir significantly suppressed SARS-CoV-2 virus titers (≥2 log TCID50/mL) in the first 24 h after infection. Repurposing of antiviral drugs is very helpful in fighting COVID-19. A safe, pan-antiviral drug such as umifenovir could be extremely beneficial in combating the early stages of a viral pandemic.

Interactions Between Child Personality and Parenting in Relation to Child Well-Being: Support for Diathesis-Stress and Differential Susceptibility Patterns.

It is well-recognized that the individual characteristics of children moderate the effects of developmental conditions on the well-being of a child. The majority of interactions follow a diathesis-stress pattern; there is also evidence for differential susceptibility and vantage sensitivity models. The present study aimed to examine interactions between parenting and child personality in relation to the well-being of a Russian child and to evaluate the models for moderated relationships. Participants were primary caregivers of 370 children aged 2-7 years. Moderation effects were examined using hierarchical multiple regression and bivariate linear models. In order to differentiate between the models of environmental sensitivity, the analysis of regions of significance was used. Consistent with a diathesis-stress framework, the results revealed that among children low in conscientiousness and high in activity, punitive parenting was a risk factor for externalizing problems; among introverted and fearful children, punitive parenting was a risk factor for internalizing problems. Positive parenting/involvement was a protective factor for internalizing behavior, only for children low in openness. The findings also demonstrate the following evidence for the differential susceptibility model: children low in Beta higher-order personality trait (also known as plasticity or personal growth) showed more total problems when faced with low positive parenting, but fewer problems when experiencing high-quality parenting.

Relations of child effortful control to personality, well-being and parenting.

This study examined effortful control and its relations to personality, parenting and well-being in a community sample of Russian preschool children (N = 365, 46% girls) using parent-reported effortful control scale from the very short form of the children's behaviour questionnaire, the inventory of child individual differences-short version, the Alabama parenting questionnaire-preschool revision, the self-reporting questionnaire, and parent and teacher reports on the strengths and difficulties questionnaire. The findings confirmed the four-factor structure of effortful control, including inhibitory control, attentional control, low-intensity pleasure and perceptual sensitivity. Girls demonstrated higher scores than boys on effortful control, perceptual sensitivity, inhibitory control and low-intensity pleasure. Older children scored higher than younger on inhibitory control and perceptual sensitivity and lower on low-intensity pleasure. Gender and age accounted for less than 3% of the variance in effortful control. Effortful control was associated with personality traits of conscientiousness, agreeableness and openness, and with positive emotions and low-negative affect. Higher effortful control was associated with higher levels of prosocial behaviour and lower levels of externalising and internalising problems. Structural modelling showed that the effect of parenting on child prosocial and problem behaviours may be mediated by effortful control.

Luminescence properties of silicon-cellulose nanocomposite.

We have characterized the structure and luminescence properties for two-component material composed of nanocrystalline cellulose and nanocrystalline (less to 100 nm) silicon powder. An efficient and stable photoluminescence of nanocomposite, resistant to the influence of gas-phase oxidants, has been found. The obtained material has electret-like properties and demonstrates the possibility of multiple-recharging in an electric field near 5·103 V/cm at temperatures ranging from -70°C to 100°C. The presence of the electric field, as well as ozone or low-temperature plasma treatment, does not change the luminescence spectrum due to quantum size properties of silicon nanoparticles. We believe that these particles may appear in two states: both embedded in a cellulose matrix and in the form of mechanical mixture.